MycoKeys 43: | | 5 30 (20 | 8) A peer-reviewed open-access journal doi: 10.3897/mycokeys.43.30776 RESEARCH ARTICLE fe) Mycokeys http://mycokeys.pensoft.net Launched to accelerate biodiversity research Two new African siblings of Pulveroboletus ravenelii (Boletaceae) Sylvestre A. Badou', André De Kesel’, Olivier Raspé”?, Martin K. Ryberg%, Atsu K. Guelly®, Nourou S. Yorou! I Research Unit Tropical Mycology and Soil-Plant-Fungi Interaction, Laboratory of Ecology, Botany and Plant Biology, Faculty of Agronomy, University of Parakou, 03 BOX: 125, Parakou, Benin 2 Meise Botanic Garden, Nieuwelaan 38, 1860 Meise, Belgium 3 Fédération Wallonie-Bruxelles, Rue A. Lavallée 1, 1080 Bruxelles, Belgium 4 Systematic Biology program, Department of Organismal Biology, Uppsala University, Norbyvagen 17D, 752 36 Uppsala, Sweden § Département de Botanique et Ecologie Végétale, Faculté des Sciences, Univer- sité de Lomé, BP1515 Lomé, Togo Corresponding author: André De Kesel (andre.dekesel@botanicgardenmeise.be) Academic editor: MP Martin | Received 23 October 2018 | Accepted 25 November 2018 | Published 12 December 2018 Citation: Badou AS, De Kesel A, Raspé O, Ryberg MK, Guelly AK, Yorou NS (2018) Two new African siblings of Pulveroboletus ravenelii (Boletaceae). MycoKeys 43: 115-130. https://doi.org/10.3897/mycokeys.43.30776 Abstract This paper sorts out the taxonomy of species affiliated with Pulveroboletus ravenelii in the Guineo-souda- nian and Zambezian woodlands of Africa. Morphological and genetic characters of African Pulveroboletus collections were studied and compared to those of North American and Asian species. A phylogenetic analysis showed that the African specimens form a subclade, sister to the Asian and American taxa. Al- though clamp connections have previously never been reported from Pulveroboletus, all specimens of the African subclade show very small clamp connections. Two new African species, Pulveroboletus africanus sp. nov. and P sokponianus sp. nov., are described and illustrated. Comments concerning morphology and identification, as well as distribution and ecology, are given for both species. Keywords Boletales, Africa, Pulveroboletus, morphology, phylogeny, taxonomy Copyright Sylvestre A. Badou et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 116 Sylvestre A. Badou et al. / MycoKeys 43: 115-130 (2018) Introduction Boletes belonging to Pulveroboletus Murrill are morphologically characterised by boletoid basidiomata with a pulverulent arachnoid veil. As originally indicated by Murrill (1909), this veil or cleistoblema sensu Clémencgon (2012), is most often yellowish to vivid yel- low and already present at a very early stage of development. Alterations broadening the circumscription of Pulveroboletus (Singer 1945, 1947, 1986), are not followed here as they have rendered the genus morphologically heterogeneous (Watling 2008) and poly- phyletic (Binder and Hibbett 2006, Wu et al. 2014, Zeng et al. 2017). In its strict sense, Pulveroboletus holds few species, all of which are similar to the type, Pulveroboletus rav- enelii (Berk. & M.A. Curtis) Murrill. Based on molecular data, Raspé et al. (2016) stated that what is called Pulveroboletus ravenelii outside North America belongs to a complex of different taxa. The name P ravenelii has been used erroneously for lookalikes in Asia, Australia (Watling 2001, Horak 2011) and also Africa (Thoen and Ducousso 1989, Wa- tling and Turnbull 1992, De Kesel et al. 2002, Yorou and De Kesel 2011, Vanié-Léabo et al. 2017, Kamou et al. 2017). So far, Raspé et al. (2016) and Zeng et al. (2017) have resolved part of the Asian complex around P ravenelii, which now counts ten species. In a similar way, this paper aims to resolve and clarify the identity of some of the African, non-viscid, Pulveroboletus that have been kept under “Pulveroboletus aff. ravenelii” . Materials and methods Sampling, microscopy and morphology Specimens were obtained from our own fieldwork or from herbarium specimens at our disposal. Protocols for field collecting, macroscopic description, drying and pres- ervation follow Eyi Ndong et al. (2011). Codes and names of colours are according to the Methuen Handbook of Colour (Kornerup and Wanscher 1978). Microscopic structures were revived and examined in 5% potassium hydroxide (KOH) or in 10% ammonia with Congo Red. Measurements and drawings of microscopic structures were undertaken using an Olympus (BX51) compound microscope equipped with digital camera and drawing tube. Dimensions of microscopic structures are presented in the following format: (a—)b—c—d(—e), in which c represents the average, b = c - 1.96 * SD and d=c+ 1.96 * SD andaand e are extreme values. Q is the length/width ratio based on at least 50 spores and is presented in the same format as spore dimensions (Eyi Ndong et al. 2011). Unless otherwise stated, herbarium specimens are deposited in BR. Duplicates from material from Togo are deposited in TOGO (Université de Lomé, Togo). Herbarium specimens from S. Badou (Benin) are deposited in UNIPAR (University of Parakou, Benin Republic). Abbreviations of herbaria follow Thiers (con- tinuously updated). MycoBank (CBS-KNAW Fungal Biodiversity Centre, continu- ously updated) numbers are provided for the new species. Two new African siblings of Pulveroboletus ravenelii (Boletaceae) LF DNA extraction, amplification and sequencing Genomic DNA was isolated from CTAB-preserved tissues or dry specimens using a CTAB isolation procedure adapted from Doyle and Doyle (1990). The genes atp6, tefl and rpb2 were amplified by PCR using the following primers: ATP6-1M40F and ATP6-2M (Raspé et al. 2016), EF1-983F and EF1-2218R (Rehner and Buck- ley 2005) and bRPB2-GF and bRPB2-7.1R (Matheny 2005). PCR products were purified by adding 1 U of Exonuclease I and 0.5 U FastAP Alkaline Phosphatase (Thermo Scientific, St. Leon-Rot, Germany) and incubated at 37 °C for 1 h, fol- lowed by inactivation at 80 °C for 15 min. Sequencing was performed by Macrogen Europe (The Netherlands) with PCR primers, except for atp6, for which universal primers M13F-pUC(-40) and M13F(-20) were used. For tefl, additional sequenc- ing was performed with two internal primers, EF1-1577F and EF1-1567R (Rehner and Buckley 2005). Alignment and phylogeny inference Sequences of Pulveroboletus species, including the type species P ravenelii, along with sequences of various genera of the Pulveroboletus group (Wu et al. 2014) and three Leccinoideae species as outgroup were generated or retrieved from GenBank (Table 1). The sequences were assembled in GENEIOUS Pro v. 6.0.6 (Biomatters). All sequenc- es were aligned using MAFFT (Katoh and Standley 2013) on the server accessed at hetp://mafft.cbre.jp/alignment/server/ and introns in rpb2 and tefl were identified based on the amino acid sequence of previously published DNA sequences. Maximum Likelihood (ML) phylogenetic tree inference was performed using RAxML (Stamata- kis 2006) on the CIPRES web server (RAxML-HPC2 on XSEDE; Miller et al. 2009). The phylogenetic tree was inferred by a single analysis with four partitions (one for the exons of each gene and a fourth for the introns of rpb2 and tef1), using the GIRCAT model with 25 categories. Statistical support of nodes was obtained with 1,000 rapid bootstrap replicates. Results DNA analyses The alignment contained sequences from 50 specimens and was 2,649 characters long (TreeBase number 23416). In the phylogram obtained (Fig. 1), Pulveroboletus formed a highly supported clade (BS = 100%). Interestingly, the African species formed a highly supported sub-clade sister to the Asian and American species, which together formed another highly supported sub-clade. 118 Sylvestre A. Badou et al. / MycoKeys 43: 115-130 (2018) lveroboletus sokponianus ADK4360 veroboletus sokponianus SAB0629 letus africanus ADK4650 oletus brunneopunctatus ORO147 oboletus brunneopunctatus HKAS55369 100 oletus brunneopunctatus HKAS74926 oboletus sp. ORO644 roboletus fragrans ORO673 eroboletus sp. ORO686 oboletus sp. ORO282 i iS ravenelii REH2565 P tus macrosporus HKAS57628 100 Suillellus subamygdalinus HKAS53641 Suillellus luridus VDKO0241b 4 Caloboletus inedulis BOTH3963 98 Caloboletus radicans VDKO1187 100 Caloboletus yunnanensis HKAS69214 Caloboletus calopus ADK4087 — Rubroboletus rhodosanguineus BOTH4263 81 Rubroboletus sinicus HKAS56304 100 Rubroboletus legaliae VDKO0936 Rubroboletus satanas VDKO0968 100 Neoboletus brunneissimus OR0249 Neoboletus brunneissimus HKAS50538 Neoboletus junquileus AF2922 8 Neoboletus magnificus HKAS54096 Neoboletus venenatus HKAS63535 100 Sutorius australiensis REH9441 Sutorius eximius REH9400 100 .- Butyriboletus roseoflavus HKAS54099 84 Butyriboletus roseoflavus ORO230 100 Butyriboletus pseudoregius VDKO0925 96 Butyriboletus appendiculatus VDKO0193b 99 400 Butyriboletus pseudospeciosus HKAS63513 Butyriboletus roseopurpureus BOTH4497 Butyriboletus subsplendidus HKAS50444 100 Rugiboletus extremiorientalis HKAS76663 100 -__L. Rugiboletus extremiorientalis ORO406 Rugiboletus brunneiporus HKAS83209 100 86 Lanmaoa angustispora HKAS74752 99 Lanmaoa asiatica HKAS63603 Lanmaoa carminipes BOTH4591 99 Cyanoboletus pulverulentus RW109 100 Cyanoboletus sp. ORO257 Cyanoboletus brunneoruber OR0233 400 Baorangia pseudocalopus HKAS75739 Baorangia pseudocalopus HKAS63607 98 Retiboletus fuscus OR0231 100 Retiboletus griseus MB03-079 ——__—— — — Rhodactina rostratispora SV208 Figure |. Maximum likelihood phylogenetic tree inferred from the three-gene dataset (atp6, rpb2, tef1), including Pulveroboletus africanus sp. nov. and Pulveroboletus sokponianus sp. nov and selected Boletaceae. The three Leccinoideae species (Retiboletus fuscus (Hongo) N.K. Zeng & Zhu L. Yang, R. griseus (Frost) Manfr. Binder & Bresinsky and Rhodactina rostratispora S. Vadthanarat, O. Raspé & S. Lumyong) were used as outgroup taxa. Bootstrap frequencies > 70% are shown above supported branches. Two new African siblings of Pulveroboletus ravenelii (Boletaceae) 119 Table |. List of collections used for DNA analyses, with origin, GenBank accession numbers and references. Species Voucher | Origin atp6 tefl rpb2 Reference Baorangia pseudocalopus HKAS63607 | China - KF112167 | KF112677 Wu et al. 2014 Baorangia pseudocalopus KJ184570 | KM605179 Wu et al. 2015 Butyriboletus appendiculatus MG212624| Vadthanarat et al. 2018 Butyriboletus pseudoregius | VDKOO0925 | Belgium |MG212538|MG212583|MG212625| Vadthanarat et al. 2018 Butyriboletus pseudospeciosus} HKAS63513 | China KT990743 | KT990380 Wu et al. 2016 Butyriboletus roseoflavus HKAS54099 KF739779 | KF739703 Wu et al. 2014 Butyriboletus roseopurpureus | BOTH4497 MG897418 | MG897428 | MG897438| Phookamsak et al. 2018 Butyriboletus subsplendidus | HKAS50444 KT990742 | KT990379 Wu et al. 2016 Butyriboletus cf. roseoflavus OR230 China | KT823974 | KT824040 | KT824007 | Raspé et al. 2016 Caloboletus calopus ADK4087 | Belgium |MG212539}| KJ184566 | KP055030 | Vadthanaratet al. 2018, Zhao et al. 2014a, 2014b Caloboletus inedulis BOTH3963 | USA |MG897414|MG897424 |MG897434| Phookamsak et al. 2018 Caloboletus radicans VDKO1187 | Belgium | MG212540|MG212584}MG212626| Vadthanarat et al. 2018 Caloboletus yunnanensis HKAS69214 | China KJ184568 | KT990396 | Zhao et al. 2014a, Wu et al. 2016 Cyanoboletus brunneoruber | OR0233 China |MG212542|]MG212586|MG212628| Vadthanarat et al. 2018 Cyanoboletus pulverulentus RW109 | Belgium Raspé et al. 2016 Cyanoboletus sp. ORO257 China Vadthanarat et al. 2018 Lanmaoa angustispora HKAS74752 | China Wu et al. 2015 Lanmaoa asiatica HKAS63603 Wu et al. 2015 Lanmaoa carminipes BOTH4591 MG897419 | MG897429 | MG897439| Phookamsak et al. 2018 Neoboletus brunneissimus | HKAS50538 | China KM605150 | KM605173 Wu et al. 2015 Neoboletus brunneissimus OR0249 China |MG212551|MG212595|MG212637| Vadthanarat et al. 2018 Neoboletus junquilleus AF2922 France |MG212552}MG212596|MG212638| Vadthanarat et al. 2018 Neoboletus magnificus HKAS54096 | China KF112149 | KF112654 Wu et al. 2014 Neoboletus venenatus KT990448 Wu et al. 2016 Pulveroboletus africanus ADK4650 KT823959 | KT824025 | KT823992 | Raspé et al. 2016 (type) Pulveroboletus ORO147 MG897420 | MG897430 | MG897440| Phookamsak et al. brunneopunctatus 2018 Pulveroboletus HKAS55369 KT990814 | KT990455 Wu et al. 2016 brunneopunctatus Pulveroboletus HKAS74926 KT990815 | KT990456 Wu et al. 2016 brunneopunctatus Pulveroboletus fragrans ORO673 ‘| Thailand} KT823977 | KT824043 | KT824010 | Raspé et al. 2016 Pulveroboletus macrosporus | HKAS57628 KT990812 | KT990453 Wu et al. 2016 Pulveroboletus sokponianus | ADK4360 KT823957 | KT824023 | KT823990 Raspé et al. 2016 (type) Pulveroboletus sokponianus | SAB0629 Benin |MH983001)/MH983002|MH983003 This study Pulveroboletus ravenelii REH2565 | U.S.A. | KU665635 | KU665636 | KU665637 | _ Raspé et al. 2016 Pulveroboletus sp. ORO282 China | MK058515 | MK058518 | MK058521 This study 120 Sylvestre A. Badou et al. / MycoKeys 43: 115-130 (2018) Species Voucher | Origin atp6 tefl rpb2 Reference Pulveroboletus sp. OR0644 | Thailand] MK058516 | MK058519 | MK058522 This study Retiboletus fuscus MG212642} Vadthanarat et al. 2018 Retiboletus griseus MBO03-079 | U.S.A. | KT823964 | KT824030 | KT823997 | Raspé et al. 2016 Rhodactina rostratispora SDBR- _ | Thailand) MG212561}MG212606|MG212646| Vadthanarat et al. CMU-SV208 2018 Rubroboletus legaliae KT824018 | Raspé et al. 2016 Rubroboletus BOTH4263 | USA |MG897416|MG897426 |MG897436| Phookamsak et al. rhodosanguineus 2018 Rubroboletus satanas VDKO0968 | Belgium | KT823986 | KT824052 | KT824019 | Raspé et al. 2016 Rubroboletus sinicus HKAS56304 | China - KJ619483 | KP055031 | Zhao et al. 2014a; fae] Zhao et al. 2014b Rugiboletus brunneiporus | HKAS83209 | China - KM605 144 | KM605168 Wu et al. 2015 Rugiboletus extremiorientalis| HKAS76663 | China - KM605147 | KM605170 Wu et al. 2015 Rugiboletus extremiorientalis| OR0406 | Thailand) MG212562|MG212607 |MG212647| Vadthanarat et al. 2018 Suillellus luridus VDKO0241b) Belgium | KT823981 | KT824047 | KT824014 Raspé et al. 2016 Suillellus subamygdalinus HKAS53641 | China KT990841 | KT990478 Wu et al. 2016 Sutorius australiensis REH9441 | Australia) MG212567 | JQ327032* |MG212652| Halling et al. 2012%*, Vadthanaratet al. 2018 Sutorius eximius REH9400 S.A. |MG212568 | JQ327029* |MG212653| Halling et al. 2012*, Vadthanaratet al. 2018 Taxonomy Pulveroboletus africanus De Kesel & Raspé, sp. nov. Mycobank MB 827970 Fig. 2a—f Illustr. Sharp (2011, page 41, ut Pulveroboletus aft. ravenelii). Holotype. Togo, Central Province, Fazao National Park, 08°44'31.9"N, 0°48'17.4"E, 6 June 2008, elev. 500 m, on the ground in a gallery forest with Berlinia grandiflora (Vahl) Hutch. & Dalziel, Uapaca guineensis Mill.-Arg. and Pandanus sp., leg. A. De Kesel, De Kesel 4650 (BR!, BR 5020165390056, duplicate in TOGO). Etymology. Epithet refers to its very wide distribution throughout tropical Africa. Description. Basidiomata medium-sized, covered by a general veil when young. Pileus 60-70 mm diam., at first broadly convex then pulvinate to plano-convex, up- per layer dark brown (GE6-GF6), dry, mat, tomentose to felty, very soon cracking, becoming tomentose-scaly, bright yellow (2A4—5) in the deeper layers, predominantly yellow with age; scales appressed, slightly fibrillose, leather brown to greyish-brown (6E4—5), thicker and darker in the centre, thinner, paler and more diffused towards the margin; margin at first incurved, appendiculate with age, vivid yellow, beset with sulphur-yellow pulverulent material. Hymenophore tubulate, separable, straight to Two new African siblings of Pulveroboletus ravenelii (Boletaceae) 121 slightly sinuate, almost free around the stipe or depressed and then only slightly decur- rent with a tooth; tubes up to 10 mm long, yellow to greyish-yellow (1B3), cyanescent when cut; pores regular, mostly round or slightly angular, slightly elongated around the stipe, small (14—-16/mm), yellow (1A2—2A2), with age greyish-brown (5E4—6), cyanescent. Stipe cylindrical, 60-80 x 8-12 mm, central, solid, uppermost part vivid yellow (2A4—5), often with some reddish fibrils and smooth, lower part sheathed from the base up with a mat, dry, fibrillose-cottony, greyish to brownish-grey (5-GEF3-—4) layer, the latter cracking transversally, forming brownish-grey to olive brown patches (4DE4-—6), first exposing a greyish-white layer, then the bright yellow deeper layers; ring at first prominent, loose membranous-cottony, vivid yellow (2AB3—5), covering the pores, later tearing, leaving fibrillose to membranous material on both pileus mar- gin and upper third of the stipe, pulverulent, becoming greenish from spores. Context yellowish-white in the pileus, marmorated yellow (1—2A2) — yellowish- white in the stipe, yellow towards the base of the stipe, cyanescent in all parts. Basal my- celium and rhizomorphs relatively thick, yellowish-white (2A2) to yellow. Odour strong fungoid. ‘Taste not recorded. Spore print greenish-olive (fresh 3D4 in Rammeloo 5720). Macrochemical reactions: tubes brown to reddish-brown with KOH and NH,OH (in collections Rammeloo 5922 and De Kesel 2163). Spores (8.4)8.6-9.5—10.3(—10.6) x (4.5)4.5-4.9-5.3(5.4) um, Q = (1.77)1.79- 1.93-2.07(2.09), broadly ellipsoid, smooth, pale yellowish-brown in 5% KOH and Melzer’s reagent, thin-walled, inamyloid. Basidia 4-spored, 22-35 x 8-12 um, clavate, hyaline, sterigmata up to 3-4 um long, without clamp connection. Cheilocystidia abundant, cylindrical to narrowly fusiform, (31.9-)32.1-42.6-53(-48.8) x (6.1- )5.6—7.2—8.7(—8.6) wm, thin-walled, hyaline. Pleurocystidia similar to cheilocystidia, not abundant. Hymenophoral trama subregular, with poorly defined mediostratum. Pileipellis a tomentum composed of irregularly arranged hyphae, the latter cylindrical, of similar shape, 3.8—5.1(6.5) um wide, slightly thick-walled (0.5 um), with brownish intracellular pigment (persistent in 5% KOH), smooth, with pulverulence in places. Stipitipellis a tomentocutis composed of elements similar to the pileipellis. Partial veil composed of cylindrical hyphae, 3-6 um wide, thin-walled and smooth. Clamp con- nections present in pileipellis tissue, small, frequent. Additional collections. BENIN, Atacora Province, Kota, 10°12.680'N, 1°26.723'E, 30 Aug. 1997, 490 m as.l., gallery forest with Berlinia grandiflora and Uapaca guineensis, De Kesel 2023 (BR 5020074869827); ibidem, 10°12.699'N, 1°26.786'E, 17 Jun. 2000, 490 m as.l., De Kesel 2824 (BR 5020126377836); ibi- dem, 10°12.665'N, 1°26.750'E, 30 Jun. 2002, 510 m a.s.l., De Kesel 3500 (BR 5020152209163); Borgou Province, Wari Maro, 9°09.884'N, 2°09.595'E, 20 Jun. 1998, 300 m a.s.l., savannah woodland with /soberlinia doka Craib & Stapf and Ua- paca togoensis Pax, De Kesel 2163 (BR 5020112674574); BURUNDI, Bururi Prov- ince, Mugara, 04°02'S, 29°31'E, 16 Nov. 1978, 1050 ma.s.l., Brachystegia woodland, Rammeloo 5720 (BR 5020019368651); ); ibid., 18 Nov. 1978, Rammeloo 5788 (BR 5020019374713); ibid., 20 Nov. 1978, Rammeloo 5811 (BR 5020032463654); ibid., 29 Nov. 1978, 950-1050 m a.s.l., Brachystegia woodland, Rammeloo 5922 122 Sylvestre A. Badou et al. / MycoKeys 43: 115-130 (2018) d e Figure 2. Pulveroboletus africanus (De Kesel 4650, holotype): a basidiomes b basidiospores € basidia d cheilocystidia e pleurocystidia f pileipellis hyphae with intracellular pigments and tiny clamps. Scale bars: 25mm (a), 10um (b), 25m (€), 25m (d, e), 50um (f). (BR 5020019378759); ibid., Rumonge-Mutambara, 4°0.756'S, 29°29.599'E, 11 Jan. 2011, 950 ma.s.l., miombo woodland with Brachystegia utilis Burtt Davy & Hutch. and B. bussei Harms, Degreef 673 (BR); GUINEA, Labé Prefecture, Fouta Djalon, N of Tountourou, 13 Jul. 1988, 1000 m a.s.l., mountain woodland with Uapaca cheva- lieri Beille, Thoen 7977 (BR 5020003130264); DR CONGO, Upper Katanga Prov- ince, near Kisangwe, Mikembo sanctuary, 11°28.790'S, 27°40.367'E, 2 Feb. 2012, Two new African siblings of Pulveroboletus ravenelii (Boletaceae) 123 1170 m a.s.l., miombo woodland with Julbernardia globiflora (Benth.) Troupin and Brachystegia spp, De Kesel 5026 (BR 5020212174363V); MALAWI, Nkhata bay dis- trict, Chisosira, 16 miles south of Chinteche, 3 Jan. 1978, woodland with Brachystegia spiciformis Benth., 450 m a.s.l., E. Tybaert 141 (BR 5020019389861, dupl. GENT); MOZAMBIQUE, Nambula Province, Natala, Reserva de Mecuburi, 27 Jan. 2011, leg. M. Harkénen, Marja Harkonen 201131 (H 7016064); TOGO, Central Prov- ince, Kparatao (towards Bassar), 09°11.630'N, 0°59.134'E, 14 Jul. 2007, 580 ma.s.l., miombo woodland with Uapaca togoensis and Monotes kerstingii Gilg., De Kesel 4359 (BR 5020163710719, duplicate in TOGO); ZIMBABWE, Midlands Province, Mtao Forest, 19°22.081'S, 30°40.383'E, 11 Feb. 1999, 1500 ma.s.L., extensively grazed mi- ombo woodland, under Brachystegia spiciformis, De Kesel 2453 (BR 5020112623060). Ethnomycological data. except for Mozambique and Zimbabwe, no local names or uses were collected. The local name in Mozambique (in Makua) is Ettuli ya Khapa (coll. Marja Hark6nen 201131), which means tortoise shell. The local name in Zimbabwe (in chiShona) is dindindi java (Sharp 2011). The species is not used for consumption. Pulveroboletus sokponianus Badou, De Kesel, Raspé & Yorou, sp. nov. Mycobank MB 827974 Fig. 3a—g Illustr. Yorou and De Kesel (2011, fig 5.10, ut Pulveroboletus ravenelii). Holotype. Togo, Central Province, Kparatao (towards Bassar), 09°11.630'N, 0°59.134'E, 14 July 2007, alt. 580 m, woodland on a slope with soberlinia doka, Uapaca togoensis and Monotes kerstingii, leg. A. De Kesel, De Kesel 4360 (BR!, BR 5020163695566, duplicate in TOGO). Etymology. in honour of the late Prof. Dr. Ir. Nestor Sokpon, esteemed colleague of the University of Parakou (Benin), for his various contributions to the understand- ing of woodland ecology and regeneration. Description. Basidiomata medium-sized, wrapped in a greenish-yellow (1A2-3) general veil when young. Pileus 40—55(60) mm diam., at first hemispherical to con- vex, then pulvinate or plano convex, upper layer pale yellow (1A2-4) to greenish-grey (1BC3-4), not cyanescent, dry, mat, tomentose to felty, becoming subtly to incon- spicuously cracked, greenish-yellow (1A2—3) in the deeper layers; scales subtle, flat, slightly felty, greenish-grey (1BC3-4), darker in the centre, diffused towards the mar- gin; margin at first incurved, appendiculate with age, greenish-yellow. Hymenophore tubulate, separable, straight to slightly sinuate, depressed around the stipe; tubes up to 7 mm long, yellow to greyish-yellow (1B3), cyanescent when cut; pores regular, mostly round or slightly angular, slightly elongated around the stipe, small (13—16/ mm), pale yellow (1A2—2A2), cyanescent. Stipe cylindrical, 42-60 x 6—7(9) mm, cen- tral, solid, uppermost part yellowish-white (1A2—3), smooth, lower part sheathed with a mat, dry, fibrillose-cottony, thick, yellowish-white to pale yellow (1A2-4) or pale greenish-grey (1BC3-—4) layer, the latter rather tearing than cracking in subtle fibrils; 124 Sylvestre A. Badou et al. / MycoKeys 43: 115-130 (2018) Figure 3. Pulveroboletus sokponianus (a, C—g De Kesel 4360, holotype, b De Kesel 3593): a basidiomes b very young basidiomes € basidiospores d basidia e cheilocystidia f pleurocystidia g pileipellis with tiny clamps. Scale bars: 25mm (a, b), 10pm (Cc), 25um (d), 25um (e, f), 50um (g). ring at first woolly, cottony, pale greenish-yellow (1A2-4), then collapsing, leaving dif- fuse remains on pileus margin and stipe, sometimes pulverulent. Context whitish to whitish-yellow in the pileus, gradually yellowish-white (1A2) towards the base of the stipe. Slightly and slowly cyanescent, except in the base of the stipe. Basal mycelium and rhizomorphs usually white. Odour fungoid, when fresh like Lepista nuda (in col- lection De Kesel 1979). Spore print and macrochemical reactions not obtained. Two new African siblings of Pulveroboletus ravenelii (Boletaceae) 125 Spores (8.5—)8.5—9.3—10.2(-10.5) x (4.4-)4.3-4.9-5.4(—5.6) um, Q = (1.76) 1.74— 1.92-2.1(—2.14), broadly ellipsoid, smooth, pale yellowish-brown in 5% KOH and Melzer’s reagent, thin-walled, inamyloid. Basidia 4-spored, 21-32 x 8-12 um, clavate, hyaline, sterigmata 3—4 um long, without clamp connection. Cheilocystidia abundant, fusiform to clavate, (36.8—)34.1-42.7-51.4(-52.8) x (6.6-)7.7-9.7-11.7(-11) um, thin-walled, with yellow intracellular pigment (persistent in NH,OH). Pleurocystidia similar to cheilocystidia, not abundant. Hymenophoral trama divergent, with regular mediostratum. Pileipellis a tomentum, composed of irregularly intertwined hyphae of similar shape, cylindrical, 3.3-5.1(6.2) um wide, entirely hyaline, smooth, with small clamps. Stipitipellis a tomentum composed of elements similar to the pileipellis. Partial veil with cylindrical hyphae, 3-6 um wide, thin-walled, smooth. Clamp connections small, frequent in the pileipellis. Additional collections. BENIN, Atacora Province, Natitingou, Kota falls, 10°12.680'N, 1°26.723'E, 23 Aug. 1997, 520 ma.s.l., savannah woodland with Jsober- linia, A. De Kesel 1979 (BR 5020074831442); ibidem, 10°12.555'N, 001°26.793'E, 26 Jun. 2004, 480 m a.s.l., forest gallery with Berlinia grandiflora and Uapaca sp., A. De Kesel 3769 (BR 5020152060610); ibidem, Kouandé, 10°17.159'N, 1°40.890'E, 25/09/2000, 470 m a.s.l., savannah woodland with Jsoberlinia tomentosa (Harms) Craib & Stapf, A. De Kesel 2942 (BR 5020129153468); ibidem, Borgou Province, Dogue, 9°07.249'N, 1°54.839'E, 10/10/2001, 350 m a.s.l., savannah woodland with Afzelia africana S.M. and Isoberlinia doka, A. De Kesel 3213 (BR 5020149693227); ibidem, Borgou Province, Okpara, 9°14.669'N, 2°43.377'E, 9 Aug. 2017, 360 ma.s.L, savannah woodland with lsoberlinia doka, S. Badou 0629 (UNIPAR); ibidem, Tama- rou (forét classée de N’dali), 9°44.680'N, 2°41.544'E, 31 Jul. 2017, 390 m a.s.l., savan- nah woodland with Lsoberlinia doka, S. Badou 0624 (UNIPAR); ibidem, 4 Aug. 2017, 390m as.l., S. Badou 0625 (UNIPAR); ibidem, Wako, 9°09.457'N, 2°05.599'E, 11/09/2001, 300 m a.s.l., savannah woodland with /soberlinia doka, Uapaca togoensis and Burkea africana Hook., A. De Kesel 3132 (BR 5020149809413); ibidem, Wari Maro, 9°10.038'N, 2°09.931'E, 20 Aug. 1997, 310 m a.s.l., savannah woodland with lsoberlinia doka, A. De Kesel 1943 (BR 5020074934501); ibidem, 9°09.884'N, 2°09.595'E, 22 Jun. 1998, 310 m as.]., savannah woodland with Jsoberlinia doka, A. De Kesel 2183 (BR 5020112693766); ibidem, 9°08.110'N, 2°10.215'E, 09/10/2001, 290 ma.s.l., savannah woodland with soberlinia doka and Uapaca togoensis, A. De Ke- sel 3188 (BR 5020149726550); ibidem, 9°09.900'N, 2°09.511'E, 23/09/2001, 310 m a.s.l., savannah woodland with /soberlinia doka and Uapaca togoensis, A. De Kesel 3237 (BR 5020149751804); ibidem, 9°10.027'N, 2°10.848'E, 16 Jun. 2002, 340 m as.l., savannah woodland with soberlinia doka and Uapaca togoensis, A. De Kesel 3411 (BR 5020152133376); ibidem, Collines Province, Toui-Kilibo, 8°32.746'N, 2°40.424'E, 19 Jul. 2017, 320 m as.|., savannah woodland with /soberlinia doka and I. tomentosa, S. Badou 0519 (UNIPAR); ibidem, 5 Jul. 2017, S. Badou 0617 (UNIPAR); ibidem, 13 Jul. 2017, S. Badou 0621 (UNIPAR); ibidem, Donga Province, Bassila, 8°57.319'N, 1°38.391'E, 14 Jun. 2002, 380 ma.s.l., savannah woodland with Berlinia grandiflora, A. De Kesel 3403 (BR 5020152245529); ibidem, 8°59.516'N, 1°38.261'E, 26 Jun. 2002, 126 Sylvestre A. Badou et al. / MycoKeys 43: 115-130 (2018) 370 ma.s.l., gallery forest with Berlinia grandiflora, Elaeis guineensis Jacq. and Raphia sp., A. De Kesel 3467 (BR 5020152045464); ibidem, 9°0.073'N, 001°39.318'E, 17 Jun. 2004, 380 m a.s.L., gallery forest with Berlinia grandiflora, A. De Kesel 3668 (BR 5020157041959); ibidem, Penessoulou (south), 9°9.688'N, 1°34.793'E, 4 Jul. 2017, 380 ma.s.L., small gallery forest with Lsoberlinia doka, S. Badou 0613 (UNIPAR); ibi- dem, 11 Aug. 2017, S. Badou 0630 (UNIPAR); ibidem, 22 Aug. 2017, S. Badou 0631 (UNIPAR); ibidem, Zou Province, Ouéssé, Gbadji forest (West side of the slope), 7°57.152'N, 001°58.095'E, 13 Jun. 2004, 310 ma.s.l., savannah woodland with /sober- linia doka, Burkea africana, A. De Kesel 3593 (BR 5020157206662). TOGO, Central Province, Fazao (Parc National), 08°42.150'N, 0°46.383’'E, 16 Jun. 2011, 520 mas.L., savannah woodland with Afzelia africana, A. De Kesel 4910 (BR 5020212173335V); ibidem, 08°43.963'N, 0°47.674'E, 16 Jul. 2007, 510 maz.s.l., savannah woodland with lsoberlinia doka and Uapaca togoensis, A. De Kesel 4382 (BR 5020163713741); ibidem, 08°38.737'N, 0°46.010'E, 17 Jul. 2007, 550 ma.s.L, gallery forest with Berlinia gran- diflora, A. De Kesel 4393 (BR 5020163839069); ibidem, 08°43.145'N, 0°46.332'E, 20 Jul. 2007, 560 m a.s.l., savannah woodland on gravelly soil, with Uapaca togoensis, A. De Kesel 4469 (BR 5020163803671); ibidem, 08°40.872'N, 0°45.487'E, 04 Jun. 2008, 680 m a.s.l., savannah woodland with Lsoberlinia doka and Uapaca togoensis, A. De Kesel 4625 (BR 5020165412277). Ethnomycological data. Except for Benin, no local names or uses were collected. The local name in Nagot language is osousou eti (coll. De Kesel 2183) and this species is not eaten. Discussion Species delimitation The African collections represent two separate species, Pulveroboletus africanus sp. nov. and P sokponianus sp. nov., both macroscopically similar to Pulveroboletus ravenelii. In the latter, however, the disc becomes reddish-orange to reddish-brown at maturity and it grows in temperate conifer woods (Bessette et al. 2016), montane Quercus forests in Costa Rica (Halling and Mueller 2005) and Colombia (Franco-Molano et al. 2000) and Pine-oak forests in the Dominican Republic/Belize (Ortiz-Santana et al. 2007). The phylogenetic analysis showed that the African specimens form a well-supported subclade within Pulveroboletus, sister to the Asian and American taxa. Although clamp connec- tions have previously been reported to be absent in Pulveroboletus (Watling 2008, Zeng et al. 2017), all specimens of the African subclade show very small clamp connections. Macroscopically, both African taxa can be distinguished based on the colour of the scales on the pileus and the stipe, being brown in P africanus and greenish-grey or yel- low in P sokponianus. In P africanus, the basal mycelium and context in the base of the stipe is generally yellow whereas it is whitish to whitish-yellow in P sokponianus. While bluing of the context depends on the freshness and the maturity of the basidiomes, it Two new African siblings of Pulveroboletus ravenelii (Boletaceae) jee seems more pronounced in P africanus. Although cystidia have been reported to be rather constant and of little use to separate Asian taxa (Zeng et al. 2017), this seems to be true for the spores of the African taxa, but not for cystidia. In P africanus, the cheilocystidia are hyaline and narrowly fusiform, whereas they are broadly fusiform and yellow pigmented in P sokponianus. Further striking characters of distinction is the brownish intracellular pigmentation in the hyphae of the pileal and stipital scales, present in P africanus but absent in P sokponianus. Young basidiomes of Pulveroboletus sokponianus are strongly reminiscent of the Asian P brunneopunctatus G. Wu & Zhu L. Yang, but the latter has a viscid veil and smaller cheilocystidia. Using the key of the Chinese species (Zeng et al. 2017), Pul veroboletus africanus approaches closest to P brunneoscabrosus Har. Takah. The latter has a viscid veil, reddish-brown scales and white to yellowish-white basal mycelium. Distribution and ecology Both new species are endemic to tropical Africa. Pulveroboletus africanus was found in Benin, Burundi, Guinea, DR Congo, Malawi, Mozambique, Togo, Zimbabwe and possibly also Zambia. It prefers regions with a pronounced wet/dry season alternance and occurs in a wide variety of woodlands, savannah woodlands and gallery forests across tropical Africa. It seems absent in the dense rainforests (Congolian region). The species is terricolous and most probably ectomycorrhizal (ECM), occurring in EcM dominated forests up to 1500 m elevation. It is difficult to ascertain if the species as- sociates with Caesalpiniaceae (Berlinia, Brachystegia, Isoberlinia, Julbernardia) and/or with Uapaca (Phyllantaceae). Only Uapaca is well represented throughout its distribu- tion range. In Eastern Africa (Zambezian region), it is also found under Brachystegia spp. and /ulbernardia spp.; in West Africa (Soudano-Guinean region) under Berlinia grandiflora and Isoberlinia spp., Thoen and Ducousso (1989) mention it under Uapaca chevalieri Beille. The species may also occur in Zambia (ut Pulveroboletus aff. ravenelii, fig. 1H in Watling and Turnbull 1992). Pulveroboletus sokponianus has so far only been found in a variety of savannah woodlands and gallery forests in the Soudano-Guinean transition zones of Benin and Togo, probably also in Ivory Coast (see fig. 3a in Léabo et al. 2017). The species is terri- colous, most probably ectomycorrhizal (EcM) and most often found under /soberlinia doka (Caesalpiniaceae). Since habitat destruction and felling of host trees is common practice in Benin, Yorou and De Kesel (2011) placed this species (mentioned as P ravenelii) under the IUCN threat category ‘vulnerable’. Acknowledgements Badou S. and Guelly A.K. acknowledge the Belgian focal point for the Global Tax- onomy Initiative (CEBioS programme) for research grants at Meise Botanic Garden 128 Sylvestre A. Badou et al. / MycoKeys 43: 115-130 (2018) (Belgium) and support for fieldwork in Togo, respectively. Badou S., Yorou N.S. and M. Ryberg are grateful to FORMAS (Sweden) for financially supporting part of the fieldwork in Benin (grant 226-2014-1109). Badou S. and Yorou N.S. thank the Volks- wagen Foundation (grant 90-127), Meise Botanic Garden and the Global Taxonomy Initiative (CEBioS programme) for financing or supplying laboratory equipment to the University of Parakou (Benin). De Kesel A. acknowledges grants and logistic support from MIKEMBO and BAK (Biodiversité au Katanga) for supporting the fieldwork in DR Congo (2012-2018). He acknowledges the King Léopold HI Fund for Nature Exploration and Conserva- tion, as well as the Foundation to Promote Scientific Research in Africa for financially supporting fieldwork in Benin (2002, 2004) and in Togo (2007, 2008, 2011). 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